2 research outputs found
Maternal Effects of Aseptic and Septic Injury on Embryonic Larval Gene Expression in the Tobacco Hornworm, Manduca Sexta
Cross-generational effects of physical and pathogenic stress have been demonstrated in several insect groups, including our model insect Manduca sexta. Prior studies in our laboratory have shown that maternal exposure to the soil-dwelling gram-negative bacteria, Serratia marcescens, just prior to adult eclosion alters egg morphology and larval immunity. Our goal is to identify mechanisms underlaying pathogen-associated parental effects on offspring. The current study advances this goal through measurement of embryonic size, embryonic histone modification, and both embryonic and larval gene expression. Two days prior to eclosion, parents were injected with saline, heat killed S. marcescens, or live S. marcescens. Embryos were collected at 24 (+/- 2) h or permitted to hatch for clearance assays (first instar) or measurement of fat body gene expression (fourth instar). We find that maternal, but not paternal, pathogen exposure significantly increases egg volume variability, and that maternal pathogen exposure may delay hatching. Furthermore, maternal injection with bacteria conferred on their offspring an enhanced ability to clear infection when compared to their saline injected peers. Histone analysis revealed that maternal treatment does not globally alter embryonic histones, however, several immune-related genes demonstrated altered expression in both embryos and fourth instar larvae
A Physiology-Driven Computational Model for Post-Cardiac Arrest Outcome Prediction
Patients resuscitated from cardiac arrest (CA) face a high risk of
neurological disability and death, however pragmatic methods are lacking for
accurate and reliable prognostication. The aim of this study was to build
computational models to predict post-CA outcome by leveraging high-dimensional
patient data available early after admission to the intensive care unit (ICU).
We hypothesized that model performance could be enhanced by integrating
physiological time series (PTS) data and by training machine learning (ML)
classifiers. We compared three models integrating features extracted from the
electronic health records (EHR) alone, features derived from PTS collected in
the first 24hrs after ICU admission (PTS24), and models integrating PTS24 and
EHR. Outcomes of interest were survival and neurological outcome at ICU
discharge. Combined EHR-PTS24 models had higher discrimination (area under the
receiver operating characteristic curve [AUC]) than models which used either
EHR or PTS24 alone, for the prediction of survival (AUC 0.85, 0.80 and 0.68
respectively) and neurological outcome (0.87, 0.83 and 0.78). The best ML
classifier achieved higher discrimination than the reference logistic
regression model (APACHE III) for survival (AUC 0.85 vs 0.70) and neurological
outcome prediction (AUC 0.87 vs 0.75). Feature analysis revealed previously
unknown factors to be associated with post-CA recovery. Results attest to the
effectiveness of ML models for post-CA predictive modeling and suggest that PTS
recorded in very early phase after resuscitation encode short-term outcome
probabilities.Comment: 51 pages, 7 figures, 4 supplementary figure